Heterologous expression of a trypanosoma cruzi surface glycoprotein (gp82) indicates that requirements for glycosylphosphatidylinositol anchoring are different in mammalian cells and this trypanosome.
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منابع مشابه
Genetic Structure and Expression of the Surface Glycoprotein GP82, the Main Adhesin of Trypanosoma cruzi Metacyclic Trypomastigotes
T. cruzi improves the likelihood of invading or adapting to the host through its capacity to present a large repertoire of surface molecules. The metacyclic stage-specific surface glycoprotein GP82 has been implicated in host cell invasion. GP82 is encoded by multiple genes from the trans-sialidase superfamily. GP82 shows a modular organization, with some variation of N-terminal region flanking...
متن کاملAdhesion of Escherichia coli to HeLa cells mediated by Trypanosoma cruzi surface glycoprotein-derived peptides inserted in the outer membrane protein LamB.
Peptides derived from the surface glycoprotein gp82 of Trypanosoma cruzi, previously implicated in the parasite's invasion of host cells, were expressed as fusions to the protein LamB of Escherichia coli in a region known to be exposed on the cell surface. Bacteria expressing these proteins adhered to HeLa cells in a manner that mimics the pattern of parasite invasion of mammalian cells. Purifi...
متن کاملOrganization and expression of a multigene family encoding the surface glycoproteins of Trypanosoma cruzi metacyclic trypomastigotes involved in the cell invasion.
The infective forms of Trypanosoma cruzi are the trypomastigote stages found in the bloodstream of mammalian hosts or the metacyclic trypomastigotes present in the digestive tract of the insect. Metacyclic trypomastigotes express two stage-specific glycoproteins (gp90 and gp82) that have no counterpart in blood trypomastigotes (Yoshida 1983, Teixeira & Yoshida 1986). The gp90 and gp82 are invol...
متن کاملMolecular Characterization of a Novel Family of Trypanosoma cruzi Surface Membrane Proteins (TcSMP) Involved in Mammalian Host Cell Invasion
BACKGROUND The surface coat of Trypanosoma cruzi is predominantly composed of glycosylphosphatidylinositol-anchored proteins, which have been extensively characterized. However, very little is known about less abundant surface proteins and their role in host-parasite interactions. METHODOLOGY/ PRINCIPAL FINDINGS Here, we described a novel family of T. cruzi surface membrane proteins (TcSMP), ...
متن کاملInfectivity of Trypanosoma cruzi strains is associated with differential expression of surface glycoproteins with differential Ca2+ signalling activity.
Mammalian cell invasion assays, using metacyclic trypomastigotes of Trypanosoma cruzi G and CL strains, showed that the CL strain enters target cells in several-fold higher numbers as compared with the G strain. Analysis of expression of surface glycoproteins in metacyclic forms of the two strains by iodination, immunoprecipitation and FACS, revealed that gp90, undetectable in the CL strain, is...
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عنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 94 4 شماره
صفحات -
تاریخ انتشار 1999